› 8qqi

Electron Microscopy
2.9Å resolution

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270688 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA gyrase subunit A Chains: A, C
Molecule details ›
Chains: A, C
Length: 524 amino acids
Theoretical weight: 58.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AES4 (Residues: 2-524; Coverage: 60%)
Gene names: JW2225, b2231, gyrA, hisW, nalA, parD
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
DNA gyrase subunit B Chains: B, D
Molecule details ›
Chains: B, D
Length: 801 amino acids
Theoretical weight: 89.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AES6 (Residues: 1-800; Coverage: 100%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains:
Mu217 chain E Chain: E
Molecule details ›
Chain: E
Length: 26 nucleotides
Theoretical weight: 7.94 KDa
Mu217 chain F Chain: F
Molecule details ›
Chain: F
Length: 26 nucleotides
Theoretical weight: 8.03 KDa

Ligands and Environments

2 bound ligands:
Ligand LRL 2 x LRL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-18592
Expression system: Escherichia coli BL21(DE3)

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