› 8qqk

Electron Microscopy
2.8Å resolution

Cryo-EM structure of E. coli cytochrome bo3 quinol oxidase assembled in peptidiscs

Released:
DOI: 10.2210/pdb8qqk/pdb
PDB entry 8qqk coloured by chain, front view.
PDB entry 8qqk coloured by chain, side view.
PDB entry 8qqk coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Cryo-EM structure of cytochrome bo3 quinol oxidase assembled in peptidiscs reveals an "open" conformation for potential ubiquinone-8 release.
Gao Y, Zhang Y, Hakke S, Mohren R, Sijbers LJPM, Peters PJ, Ravelli RBG
Biochim Biophys Acta Bioenerg 149045 (2024)
PMID: 38614453
Related structures: EMD-18594

Function and Biology Details

Reaction catalysed: 
2 ubiquinol + O(2) + n H(+)(Side 1) = 2 ubiquinone + 2 H(2)O + n H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142171 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cytochrome bo(3) ubiquinol oxidase subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 663 amino acids
Theoretical weight: 74.42 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABI8 (Residues: 1-663; Coverage: 100%)
Gene names: JW0421, b0431, cyoB
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome bo(3) ubiquinol oxidase subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 315 amino acids
Theoretical weight: 34.95 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABJ1 (Residues: 1-315; Coverage: 100%)
Gene names: JW0422, b0432, cyoA
Sequence domains:
Cytochrome bo(3) ubiquinol oxidase subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 204 amino acids
Theoretical weight: 22.64 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABJ3 (Residues: 1-204; Coverage: 100%)
Gene names: JW0420, b0430, cyoC
Sequence domains: Cytochrome c oxidase subunit III
Cytochrome bo(3) ubiquinol oxidase subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 109 amino acids
Theoretical weight: 12.04 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABJ6 (Residues: 1-109; Coverage: 100%)
Gene names: JW0419, b0429, cyoD
Sequence domains: Prokaryotic Cytochrome C oxidase subunit IV

Ligands and Environments

5 bound ligands:
Ligand POV 3 x POV
Ligand 3PE 3 x 3PE
Ligand HEM 1 x HEM
Ligand HEO 1 x HEO
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-18594

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