8qt4

X-ray diffraction
1.55Å resolution

Crystal structure of human Sirt2 in complex with the super-slow substrate TNFn-6 and NAD+

Released:
Model geometry
Fit model/data
Source organism: Homo sapiens
Entry authors: Friedrich F, Kalbas D, Meleshin M, Einsle O, Schutkowski M, Jung M

Function and Biology Details

Reaction catalysed:
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-280078 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacetylase sirtuin-2 Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Peptide-based super-slow substrate TNFn-6 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.01 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 38.056Å b: 74.847Å c: 55.981Å
α: 90° β: 96.37° γ: 90°
R-values:
R R work R free
0.16 0.159 0.188
Expression systems:
  • Escherichia coli
  • Not provided