› 8qti

Electron Microscopy
3.09Å resolution

Mycobacterium smegnatis RNAP open promoter complex with SigmaA and RbpA

Released:
DOI: 10.2210/pdb8qti/pdb
PDB entry 8qti coloured by chain, front view.
PDB entry 8qti coloured by chain, side view.
PDB entry 8qti coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Mycobacterial HelD connects RNA polymerase recycling with transcription initiation.
Kovaľ T, Borah N, Sudzinová P, Brezovská B, Šanderová H, Vaňková Hausnerová V, Křenková A, Hubálek M, Trundová M, Adámková K, Dušková J, Schwarz M, Wiedermannová J, Dohnálek J, Krásný L, Kouba T
Nat Commun 15 8740 (2024)
PMID: 39384756
Related structures: EMD-18650

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-282673 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 37.96 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QSL8 (Residues: 1-350; Coverage: 100%)
Gene names: MSMEG_1524, MSMEI_1488, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1169 amino acids
Theoretical weight: 128.68 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: P60281 (Residues: 1-1169; Coverage: 100%)
Gene names: MSMEG_1367, MSMEI_1328, rpoB
Sequence domains:
RNA polymerase sigma factor SigA Chain: F
DNA-directed RNA polymerase subunit beta' Chain: D
DNA-directed RNA polymerase subunit omega Chain: E
RNA polymerase-binding protein RbpA Chain: J
DNA 50-mer non-template strand Chain: O
DNA 50-mer template strand Chain: P

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.09Å
Relevant EMDB volumes: EMD-18650
Expression system: Escherichia coli

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