› 8qu8

Electron Microscopy
3.5Å resolution

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236255 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
von Hippel-Lindau disease tumor suppressor Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 24.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40337 (Residues: 2-213; Coverage: 100%)
Gene name: VHL
Sequence domains:
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 117 amino acids
Theoretical weight: 13.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15370 (Residues: 2-118; Coverage: 99%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 111 amino acids
Theoretical weight: 12.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15369 (Residues: 2-112; Coverage: 99%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Cullin-2 Chain: D
Molecule details ›
Chain: D
Length: 744 amino acids
Theoretical weight: 86.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13617 (Residues: 2-745; Coverage: 100%)
Gene name: CUL2
Sequence domains:
E3 ubiquitin-protein ligase RBX1 Chain: E
Molecule details ›
Chain: E
Length: 107 amino acids
Theoretical weight: 12.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62877 (Residues: 2-108; Coverage: 99%)
Gene names: RBX1, RNF75, ROC1
Sequence domains: RING-H2 zinc finger domain
GTPase KRas, N-terminally processed Chain: F
Molecule details ›
Chain: F
Length: 170 amino acids
Theoretical weight: 19.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-164; Coverage: 87%)
  • Best match: P01116-2 (Residues: 1-164)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand GDP 1 x GDP
Ligand WYL 1 x WYL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-18657
Expression system: Escherichia coli

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