› 8qv8

Electron Microscopy
3.26Å resolution

Cryo-EM structure of the heat-irreversible amyloid fibrils of hen egg-white lysozyme

Released:
DOI: 10.2210/pdb8qv8/pdb
PDB entry 8qv8 coloured by chain, front view.
PDB entry 8qv8 coloured by chain, side view.
PDB entry 8qv8 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
A structural rationale for reversible vs irreversible amyloid fibril formation from a single protein.
Frey L, Zhou J, Cereghetti G, Weber ME, Rhyner D, Pokharna A, Wenchel L, Kadavath H, Cao Y, Meier BH, Peter M, Greenwald J, Riek R, Mezzenga R
Nat Commun 15 8448 (2024)
PMID: 39349464
Related structures: EMD-18669

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279399 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lysozyme C Chains: A, C, E, G, I
Molecule details ›
Chains: A, C, E, G, I
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Unidentified peptide Chains: B, D, F, H, J
Molecule details ›
Chains: B, D, F, H, J
Length: 9 amino acids
Theoretical weight: 784 Da
Source organism: Gallus gallus
Expression system: Gallus gallus
Unidentified peptide Chains: K, L, M, N, O

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.26Å
Relevant EMDB volumes: EMD-18669
Expression system: Gallus gallus

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