› 8qxv

Electron Microscopy
13.6Å resolution

In situ structure average of GroEL14-GroES7 complexes with narrow GroEL7 trans ring conformation in Escherichia coli cytosol obtained by cryo electron tomography

Released:
DOI: 10.2210/pdb8qxv/pdb
PDB entry 8qxv coloured by chain, front view.
PDB entry 8qxv coloured by chain, side view.
PDB entry 8qxv coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Visualizing chaperonin function in situ by cryo-electron tomography.
Wagner J, Carvajal AI, Bracher A, Beck F, Wan W, Bohn S, Körner R, Baumeister W, Fernandez-Busnadiego R, Hartl FU
Nature (2024)
PMID: 39169181
Related structures: EMD-18738

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141318 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 547 amino acids
Theoretical weight: 57.26 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6F5 (Residues: 2-548; Coverage: 100%)
Gene names: JW4103, b4143, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Co-chaperonin GroES Chains: O, P, Q, R, S, T, U
Molecule details ›
Chains: O, P, Q, R, S, T, U
Length: 97 amino acids
Theoretical weight: 10.4 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6F9 (Residues: 1-97; Coverage: 100%)
Gene names: JW4102, b4142, groES, groS, mopB
Sequence domains: Chaperonin 10 Kd subunit

Ligands and Environments

4 bound ligands:
Ligand ATP 7 x ATP
Ligand MG 14 x MG
Ligand K 14 x K
Ligand ADP 7 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 13.6Å
Relevant EMDB volumes: EMD-18738
Expression system: Escherichia coli BL21(DE3)

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