› 8qy1

X-ray diffraction
1.9Å resolution

Xylanase from Bacillus circulans mutant E78Q/Y69A bound to xylohexaose

Released:
DOI: 10.2210/pdb8qy1/pdb
PDB entry 8qy1 coloured by chain, front view.
PDB entry 8qy1 coloured by chain, side view.
PDB entry 8qy1 coloured by chain, top view.
Source organism: Niallia circulans subsp. circulans
Primary publication:
Bimodal substrate binding in the active site of the glycosidase BcX.
Saberi M, Chikunova A, Ben Bdira F, Cramer-Blok A, Timmer M, Voskamp P, Ubbink M
FEBS J (2024)
PMID: 39185686

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Endo-1,4-beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 20.32 KDa
Source organism: Niallia circulans subsp. circulans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09850 (Residues: 29-213; Coverage: 100%)
Gene name: xlnA
Sequence domains: Glycosyl hydrolases family 11

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
3 bound ligands:
Ligand ZN 2 x ZN
Ligand BTB 2 x BTB
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P43212
Unit cell:
a: 78.05Å b: 78.05Å c: 133.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.238
Expression system: Escherichia coli BL21(DE3)

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