› 8qyi

X-ray diffraction
2.3Å resolution

OleP in complex with lithocholic acid in high salt crystallization conditions

Released:
DOI: 10.2210/pdb8qyi/pdb
PDB entry 8qyi coloured by chain, front view.
PDB entry 8qyi coloured by chain, side view.
PDB entry 8qyi coloured by chain, top view.
Source organism: Streptomyces antibioticus
Entry authors: Fata F, Costanzo A, Freda I, Gugole E, Bulfaro G, Barbizzi L, Di Renzo M, Savino C, Vallone B, Montemiglio LC

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P-450 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 398 amino acids
Theoretical weight: 44.07 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59819 (Residues: 10-407; Coverage: 98%)
Gene name: oleP
Sequence domains: Cytochrome P450

Ligands and Environments

4 bound ligands:
Ligand HEM 6 x HEM
Ligand 4OA 6 x 4OA
Ligand FMT 102 x FMT
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: C2
Unit cell:
a: 249.373Å b: 109.269Å c: 160.658Å
α: 90° β: 129.6° γ: 90°
R-values:
R R work R free
0.176 0.173 0.222
Expression system: Escherichia coli BL21(DE3)

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