› 8qyn

Electron Microscopy
2.88Å resolution

Human 20S proteasome assembly intermediate structure 5

Released:
DOI: 10.2210/pdb8qyn/pdb
PDB entry 8qyn coloured by chain, front view.
PDB entry 8qyn coloured by chain, side view.
PDB entry 8qyn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Visualizing chaperone-mediated multistep assembly of the human 20S proteasome.
Adolf F, Du J, Goodall EA, Walsh RM, Rawson S, von Gronau S, Harper JW, Hanna J, Schulman BA
Nat Struct Mol Biol (2024)
PMID: 38600324
Related structures: EMD-18759

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271556 (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
Proteasome subunit alpha type-2 Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chain: C
Molecule details ›
Chain: C
Length: 248 amino acids
Theoretical weight: 27.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O14818 (Residues: 1-248; Coverage: 100%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chain: D
Molecule details ›
Chain: D
Length: 242 amino acids
Theoretical weight: 26.46 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28066 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chain: E
Molecule details ›
Chain: E
Length: 264 amino acids
Theoretical weight: 29.62 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25786 (Residues: 1-263; Coverage: 100%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chain: F
Molecule details ›
Chain: F
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit alpha type-6 Chain: G
Molecule details ›
Chain: G
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome maturation protein Chain: H
Molecule details ›
Chain: H
Length: 142 amino acids
Theoretical weight: 15.83 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9Y244 (Residues: 1-141; Coverage: 100%)
Gene names: C13orf12, HSPC014, HSPC036, PNAS-110, POMP, UMP1
Sequence domains: Proteasome maturation factor UMP1
Proteasome assembly chaperone 1 Chain: I
Molecule details ›
Chain: I
Length: 288 amino acids
Theoretical weight: 32.89 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O95456 (Residues: 1-288; Coverage: 100%)
Gene names: C21LRP, DSCR2, PAC1, PSMG1
Sequence domains: Proteasome assembly chaperone 4
Proteasome assembly chaperone 2 Chain: J
Molecule details ›
Chain: J
Length: 265 amino acids
Theoretical weight: 29.45 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q969U7 (Residues: 1-264; Coverage: 100%)
Gene names: HCCA3, PAC2, PSMG2, TNFSF5IP1
Sequence domains: PAC2 family
Proteasome subunit beta type-7 Chain: K
Molecule details ›
Chain: K
Length: 332 amino acids
Theoretical weight: 35.49 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q99436 (Residues: 1-277; Coverage: 100%)
Gene names: PSMB7, Z
Sequence domains:
Proteasome subunit beta type-3 Chain: L
Molecule details ›
Chain: L
Length: 205 amino acids
Theoretical weight: 22.97 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49720 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chain: M
Molecule details ›
Chain: M
Length: 202 amino acids
Theoretical weight: 22.89 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49721 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chain: N
Molecule details ›
Chain: N
Length: 264 amino acids
Theoretical weight: 28.54 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28074 (Residues: 1-263; Coverage: 100%)
Gene names: LMPX, MB1, PSMB5, X
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chain: O
Molecule details ›
Chain: O
Length: 241 amino acids
Theoretical weight: 26.52 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P20618 (Residues: 1-241; Coverage: 100%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chain: P
Molecule details ›
Chain: P
Length: 264 amino acids
Theoretical weight: 29.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28070 (Residues: 1-264; Coverage: 100%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit
Proteasome subunit beta type-6 Chain: Q
Molecule details ›
Chain: Q
Length: 239 amino acids
Theoretical weight: 25.34 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28072 (Residues: 1-239; Coverage: 100%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-18759
Expression system: Trichoplusia ni

Quick links

Citations

2 citation in other articles

Structural basis of human 20S proteasome biogenesis.
Zhang et al. (2024)
1 more

1 mention without citation

Visualizing chaperone-mediated multistep assembly of the human 20S proteasome.
Adolf et al. (2024)