8qyn

Electron Microscopy
2.88Å resolution

Human 20S proteasome assembly intermediate structure 5

Released:
Source organism: Homo sapiens
Related structures: EMD-18759

Function and Biology Details

Reaction catalysed:
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
PDBe Complex ID:
PDB-CPX-271556 (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
Proteasome subunit alpha type-2 Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chain: C
Molecule details ›
Chain: C
Length: 248 amino acids
Theoretical weight: 27.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O14818 (Residues: 1-248; Coverage: 100%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chain: D
Molecule details ›
Chain: D
Length: 242 amino acids
Theoretical weight: 26.46 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28066 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chain: E
Molecule details ›
Chain: E
Length: 264 amino acids
Theoretical weight: 29.62 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25786 (Residues: 1-263; Coverage: 100%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chain: F
Molecule details ›
Chain: F
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit alpha type-6 Chain: G
Molecule details ›
Chain: G
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome maturation protein Chain: H
Molecule details ›
Chain: H
Length: 142 amino acids
Theoretical weight: 15.83 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9Y244 (Residues: 1-141; Coverage: 100%)
Gene names: C13orf12, HSPC014, HSPC036, PNAS-110, POMP, UMP1
Sequence domains: Proteasome maturation factor UMP1
Proteasome assembly chaperone 1 Chain: I
Molecule details ›
Chain: I
Length: 288 amino acids
Theoretical weight: 32.89 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O95456 (Residues: 1-288; Coverage: 100%)
Gene names: C21LRP, DSCR2, PAC1, PSMG1
Sequence domains: Proteasome assembly chaperone 4
Proteasome assembly chaperone 2 Chain: J
Molecule details ›
Chain: J
Length: 265 amino acids
Theoretical weight: 29.45 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q969U7 (Residues: 1-264; Coverage: 100%)
Gene names: HCCA3, PAC2, PSMG2, TNFSF5IP1
Sequence domains: PAC2 family
Proteasome subunit beta type-7 Chain: K
Molecule details ›
Chain: K
Length: 332 amino acids
Theoretical weight: 35.49 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q99436 (Residues: 1-277; Coverage: 100%)
Gene names: PSMB7, Z
Sequence domains:
Proteasome subunit beta type-3 Chain: L
Molecule details ›
Chain: L
Length: 205 amino acids
Theoretical weight: 22.97 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49720 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chain: M
Molecule details ›
Chain: M
Length: 202 amino acids
Theoretical weight: 22.89 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49721 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chain: N
Molecule details ›
Chain: N
Length: 264 amino acids
Theoretical weight: 28.54 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28074 (Residues: 1-263; Coverage: 100%)
Gene names: LMPX, MB1, PSMB5, X
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chain: O
Molecule details ›
Chain: O
Length: 241 amino acids
Theoretical weight: 26.52 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P20618 (Residues: 1-241; Coverage: 100%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chain: P
Molecule details ›
Chain: P
Length: 264 amino acids
Theoretical weight: 29.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28070 (Residues: 1-264; Coverage: 100%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit
Proteasome subunit beta type-6 Chain: Q
Molecule details ›
Chain: Q
Length: 239 amino acids
Theoretical weight: 25.34 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28072 (Residues: 1-239; Coverage: 100%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-18759
Expression system: Trichoplusia ni