› 8qyo

Electron Microscopy
2.84Å resolution

Human proteasome 20S core particle

Released:
DOI: 10.2210/pdb8qyo/pdb
PDB entry 8qyo coloured by chain, front view.
PDB entry 8qyo coloured by chain, side view.
PDB entry 8qyo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Visualizing chaperone-mediated multistep assembly of the human 20S proteasome.
Adolf F, Du J, Goodall EA, Walsh RM, Rawson S, von Gronau S, Harper JW, Hanna J, Schulman BA
Nat Struct Mol Biol (2024)
PMID: 38600324
Related structures: EMD-18760

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127173 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit alpha type-2 Chains: A, O
Molecule details ›
Chains: A, O
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chains: B, P
Molecule details ›
Chains: B, P
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chains: C, Q
Proteasome subunit alpha type-5 Chains: D, R
Proteasome subunit alpha type-1 Chains: E, S
Proteasome subunit alpha type-3 Chains: F, T
Proteasome subunit alpha type-6 Chains: G, U
Proteasome subunit beta type-7 Chains: H, V
Proteasome subunit beta type-3 Chains: I, W
Proteasome subunit beta type-2 Chains: J, X
Proteasome subunit beta type-5 Chains: K, Y
Proteasome subunit beta type-1 Chains: L, Z
Proteasome subunit beta type-4 Chains: M, a
Proteasome subunit beta type-6 Chains: N, b

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-18760
Expression system: Trichoplusia ni

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