› 8qz3

X-ray diffraction
2.4Å resolution

Crystal structure of human two pore domain potassium ion channel TREK-2 (K2P10.1) in complex with an activatory nanobody (Nb67)

Released:
DOI: 10.2210/pdb8qz3/pdb
PDB entry 8qz3 coloured by chain, front view.
PDB entry 8qz3 coloured by chain, side view.
PDB entry 8qz3 coloured by chain, top view.
Source organisms:
Primary publication:
Extracellular modulation of TREK-2 activity with nanobodies provides insight into the mechanisms of K2P channel regulation.
Rödström KEJ, Cloake A, Sörmann J, Baronina A, Smith KHM, Pike ACW, Ang J, Proks P, Schewe M, Holland-Kaye I, Bushell SR, Elliott J, Pardon E, Baukrowitz T, Owens RJ, Newstead S, Steyaert J, Carpenter EP, Tucker SJ
OpenAccess logo Nat Commun 15 4173 (2024)
PMID: 38755204

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244818 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Potassium channel subfamily K member 10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 30.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Nanobody 67 Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 137 amino acids
Theoretical weight: 14.94 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand K 5 x K
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Unit cell:
a: 51.264Å b: 102.1Å c: 215.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.278 0.291
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Effect of two activators on the gating of a K<sub>2P</sub> channel.
Mendez-Otalvaro et al. (2024)