› 8qzj

X-ray diffraction
2Å resolution

Crystal structure of PptT-ADP from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb8qzj/pdb
PDB entry 8qzj coloured by chain, front view.
PDB entry 8qzj coloured by chain, side view.
PDB entry 8qzj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Catalytic Cycle of Type II 4'-Phosphopantetheinyl Transferases
Gavalda S, Faille A, Fioccola S, Nguyen MC, Carivenc C, Rottier K, Rufin Y, Saitta S, Czaplicki G, Guilhot C, Chalut C, Brut M, Mourey L, Pedelacq JD
ACS Catalysis 14 8561-8575 (2024)

Function and Biology Details

Reaction catalysed: 
CoA-(4'-phosphopantetheine) + an apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128483 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4'-phosphopantetheinyl transferase PptT Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 26.91 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O33336 (Residues: 1-227; Coverage: 100%)
Gene names: Rv2794c, pptT
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand A3P 4 x A3P
Ligand MN 8 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Unit cell:
a: 65.86Å b: 68.792Å c: 210.063Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.219 0.268
Expression system: Escherichia coli BL21(DE3)

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