› 8qzn

X-ray diffraction
1.4Å resolution

DARPin-Armadillo fusion protein with a selected Tyrosin pocket binding to the fused target peptide

Released:
DOI: 10.2210/pdb8qzn/pdb
PDB entry 8qzn coloured by chain, front view.
PDB entry 8qzn coloured by chain, side view.
PDB entry 8qzn coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Modular binder technology by NGS-aided, high-resolution selection in yeast of designed armadillo modules.
Stark Y, Menard F, Jeliazkov JR, Ernst P, Chembath A, Ashraf M, Hine AV, Plückthun A
Proc Natl Acad Sci U S A 121 e2318198121 (2024)
PMID: 38917007

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DARPin-Armadillo fusion protein with Tyrosin pocket and linked target peptide Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 36.57 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand EDO 15 x EDO
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 50.85Å b: 93.61Å c: 76.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.18
Expression system: Escherichia coli

Quick links