Function and Biology Details
Reactions catalysed:
ATP + H(2)O = ADP + phosphate
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure analysis Details
Assembly composition:
hetero 60-mer (preferred)
PDBe Complex ID:
PDB-CPX-244529 (preferred)
Entry contents:
40 distinct polypeptide molecules
6 distinct RNA molecules
6 distinct RNA molecules
Macromolecules (46 distinct):
Pre-mRNA-processing-splicing factor 8
Chain: A
Molecule details ›
Chain: A
Length: 2335 amino acids
Theoretical weight: 273.97 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 2335 amino acids
Theoretical weight: 273.97 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q6P2Q9 (Residues: 1-2335; Coverage: 100%)
Sequence domains:
U5 small nuclear ribonucleoprotein 200 kDa helicase
Chain: B
Molecule details ›
Splicing factor 3A subunit 1
Chain: 7
Molecule details ›
Chain: 7
Length: 793 amino acids
Theoretical weight: 88.99 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 793 amino acids
Theoretical weight: 88.99 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q15459 (Residues: 1-793; Coverage: 100%)
Sequence domains:
Splicing factor 3A subunit 2
Chain: 8
Molecule details ›
Chain: 8
Length: 464 amino acids
Theoretical weight: 49.33 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 464 amino acids
Theoretical weight: 49.33 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q15428 (Residues: 1-464; Coverage: 100%)
Sequence domains:
116 kDa U5 small nuclear ribonucleoprotein component
Chain: C
Molecule details ›
Chain: C
Length: 972 amino acids
Theoretical weight: 109.56 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 972 amino acids
Theoretical weight: 109.56 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q15029 (Residues: 1-972; Coverage: 100%)
Sequence domains:
Thioredoxin-like protein 4A
Chain: D
Molecule details ›
Chain: D
Length: 142 amino acids
Theoretical weight: 16.81 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: Mitosis protein DIM1
Length: 142 amino acids
Theoretical weight: 16.81 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P83876 (Residues: 1-142; Coverage: 100%)
Sequence domains: Mitosis protein DIM1
U5 small nuclear ribonucleoprotein 40 kDa protein
Chain: E
Molecule details ›
Chain: E
Length: 357 amino acids
Theoretical weight: 39.36 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: WD domain, G-beta repeat
Length: 357 amino acids
Theoretical weight: 39.36 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q96DI7 (Residues: 1-357; Coverage: 100%)
Sequence domains: WD domain, G-beta repeat
Probable ATP-dependent RNA helicase DDX23
Chain: G
Molecule details ›
Chain: G
Length: 820 amino acids
Theoretical weight: 95.79 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 820 amino acids
Theoretical weight: 95.79 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q9BUQ8 (Residues: 1-820; Coverage: 100%)
Sequence domains:
NHP2-like protein 1
Chain: M
Molecule details ›
Chain: M
Length: 128 amino acids
Theoretical weight: 14.19 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Length: 128 amino acids
Theoretical weight: 14.19 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P55769 (Residues: 1-128; Coverage: 100%)
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Ubiquitin carboxyl-terminal hydrolase 39
Chain: U
Molecule details ›
Peptidyl-prolyl cis-trans isomerase H
Chain: W
Molecule details ›
Chain: W
Length: 177 amino acids
Theoretical weight: 19.23 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Length: 177 amino acids
Theoretical weight: 19.23 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
O43447 (Residues: 1-177; Coverage: 100%)
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
U6 snRNA-associated Sm-like protein LSm6
Chain: 66
Molecule details ›
Chain: 66
Length: 80 amino acids
Theoretical weight: 9.14 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 80 amino acids
Theoretical weight: 9.14 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P62312 (Residues: 1-80; Coverage: 100%)
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm7
Chain: 67
Molecule details ›
Chain: 67
Length: 103 amino acids
Theoretical weight: 11.62 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 103 amino acids
Theoretical weight: 11.62 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q9UK45 (Residues: 1-103; Coverage: 100%)
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm2
Chain: 62
Molecule details ›
Chain: 62
Length: 95 amino acids
Theoretical weight: 10.85 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 95 amino acids
Theoretical weight: 10.85 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q9Y333 (Residues: 1-95; Coverage: 100%)
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm3
Chain: 63
Molecule details ›
Chain: 63
Length: 102 amino acids
Theoretical weight: 11.86 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 102 amino acids
Theoretical weight: 11.86 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P62310 (Residues: 1-102; Coverage: 100%)
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm8
Chain: 68
Molecule details ›
Chain: 68
Length: 96 amino acids
Theoretical weight: 10.41 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 96 amino acids
Theoretical weight: 10.41 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
O95777 (Residues: 1-96; Coverage: 100%)
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm4
Chain: 64
Molecule details ›
Chain: 64
Length: 139 amino acids
Theoretical weight: 15.38 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 139 amino acids
Theoretical weight: 15.38 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q9Y4Z0 (Residues: 1-139; Coverage: 100%)
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm5
Chain: 65
Molecule details ›
Chain: 65
Length: 91 amino acids
Theoretical weight: 9.95 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 91 amino acids
Theoretical weight: 9.95 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q9Y4Y9 (Residues: 1-91; Coverage: 100%)
Sequence domains: LSM domain
Splicing factor 3B subunit 4
Chain: B4
Molecule details ›
Chain: B4
Length: 424 amino acids
Theoretical weight: 44.44 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: RNA recognition motif
Length: 424 amino acids
Theoretical weight: 44.44 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q15427 (Residues: 1-424; Coverage: 100%)
Sequence domains: RNA recognition motif
Splicing factor 3A subunit 3
Chain: 9
Molecule details ›
Chain: 9
Length: 501 amino acids
Theoretical weight: 58.93 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 501 amino acids
Theoretical weight: 58.93 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q12874 (Residues: 1-501; Coverage: 100%)
Sequence domains:
Splicing factor 3B subunit 2
Chain: B2
Molecule details ›
Chain: B2
Length: 895 amino acids
Theoretical weight: 100.38 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 895 amino acids
Theoretical weight: 100.38 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q13435 (Residues: 1-895; Coverage: 100%)
Sequence domains:
Splicing factor 3B subunit 5
Chain: B5
Molecule details ›
Chain: B5
Length: 86 amino acids
Theoretical weight: 10.15 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: Splicing factor 3B subunit 10 (SF3b10)
Length: 86 amino acids
Theoretical weight: 10.15 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q9BWJ5 (Residues: 1-86; Coverage: 100%)
Sequence domains: Splicing factor 3B subunit 10 (SF3b10)
Splicing factor 3B subunit 3
Chain: B3
Molecule details ›
Chain: B3
Length: 1217 amino acids
Theoretical weight: 135.72 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 1217 amino acids
Theoretical weight: 135.72 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q15393 (Residues: 1-1217; Coverage: 100%)
Sequence domains:
PHD finger-like domain-containing protein 5A
Chain: BP
Molecule details ›
Chain: BP
Length: 110 amino acids
Theoretical weight: 12.43 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: PHF5-like protein
Length: 110 amino acids
Theoretical weight: 12.43 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q7RTV0 (Residues: 1-110; Coverage: 100%)
Sequence domains: PHF5-like protein
Splicing factor 3B subunit 1
Chain: B1
Molecule details ›
Chain: B1
Length: 1304 amino acids
Theoretical weight: 146.02 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 1304 amino acids
Theoretical weight: 146.02 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
O75533 (Residues: 1-1304; Coverage: 100%)
Sequence domains:
Splicing factor 3B subunit 6
Chain: B6
Molecule details ›
Chain: B6
Length: 125 amino acids
Theoretical weight: 14.61 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: RNA recognition motif
Length: 125 amino acids
Theoretical weight: 14.61 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q9Y3B4 (Residues: 1-125; Coverage: 100%)
Sequence domains: RNA recognition motif
Small nuclear ribonucleoprotein Sm D2
Chains: 22, 42, 52
Molecule details ›
Chains: 22, 42, 52
Length: 118 amino acids
Theoretical weight: 13.55 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 118 amino acids
Theoretical weight: 13.55 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P62316 (Residues: 1-118; Coverage: 100%)
Sequence domains: LSM domain
U2 small nuclear ribonucleoprotein B''
Chain: 2B
Molecule details ›
Chain: 2B
Length: 225 amino acids
Theoretical weight: 25.52 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: RNA recognition motif
Length: 225 amino acids
Theoretical weight: 25.52 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P08579 (Residues: 1-225; Coverage: 100%)
Sequence domains: RNA recognition motif
Small nuclear ribonucleoprotein F
Chains: 2f, 4f, 5f
Molecule details ›
Chains: 2f, 4f, 5f
Length: 86 amino acids
Theoretical weight: 9.73 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 86 amino acids
Theoretical weight: 9.73 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P62306 (Residues: 1-86; Coverage: 100%)
Sequence domains: LSM domain
Small nuclear ribonucleoprotein-associated proteins B and B'
Chains: 2b, 4b, 5b
Molecule details ›
Chains: 2b, 4b, 5b
Length: 240 amino acids
Theoretical weight: 24.64 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 240 amino acids
Theoretical weight: 24.64 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P14678 (Residues: 1-240; Coverage: 100%)
Sequence domains: LSM domain
Small nuclear ribonucleoprotein Sm D3
Chains: 23, 43, 53
Molecule details ›
Chains: 23, 43, 53
Length: 126 amino acids
Theoretical weight: 13.94 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 126 amino acids
Theoretical weight: 13.94 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P62318 (Residues: 1-126; Coverage: 100%)
Sequence domains: LSM domain
Small nuclear ribonucleoprotein G
Chains: 2g, 4g, 5g
Molecule details ›
Chains: 2g, 4g, 5g
Length: 76 amino acids
Theoretical weight: 8.51 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 76 amino acids
Theoretical weight: 8.51 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P62308 (Residues: 1-76; Coverage: 100%)
Sequence domains: LSM domain
Small nuclear ribonucleoprotein E
Chains: 2e, 4e, 5e
Molecule details ›
Chains: 2e, 4e, 5e
Length: 92 amino acids
Theoretical weight: 10.82 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 92 amino acids
Theoretical weight: 10.82 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P62304 (Residues: 1-92; Coverage: 100%)
Sequence domains: LSM domain
Small nuclear ribonucleoprotein Sm D1
Chains: 21, 41, 51
Molecule details ›
Chains: 21, 41, 51
Length: 119 amino acids
Theoretical weight: 13.31 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: LSM domain
Length: 119 amino acids
Theoretical weight: 13.31 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P62314 (Residues: 1-119; Coverage: 100%)
Sequence domains: LSM domain
U2 small nuclear ribonucleoprotein A'
Chain: 2A
Molecule details ›
Chain: 2A
Length: 255 amino acids
Theoretical weight: 28.46 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains: Leucine-rich repeat
Length: 255 amino acids
Theoretical weight: 28.46 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P09661 (Residues: 1-255; Coverage: 100%)
Sequence domains: Leucine-rich repeat
U4/U6 small nuclear ribonucleoprotein Prp4
Chain: F
Molecule details ›
Chain: F
Length: 522 amino acids
Theoretical weight: 58.54 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 522 amino acids
Theoretical weight: 58.54 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
O43172 (Residues: 1-522; Coverage: 100%)
Sequence domains:
U4/U6 small nuclear ribonucleoprotein Prp3
Chain: J
Molecule details ›
Chain: J
Length: 683 amino acids
Theoretical weight: 77.67 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 683 amino acids
Theoretical weight: 77.67 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
O43395 (Residues: 1-683; Coverage: 100%)
Sequence domains:
U4/U6 small nuclear ribonucleoprotein Prp31
Chain: L
Molecule details ›
Chain: L
Length: 499 amino acids
Theoretical weight: 55.53 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 499 amino acids
Theoretical weight: 55.53 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
Q8WWY3 (Residues: 1-499; Coverage: 100%)
Sequence domains:
Pre-mRNA-processing factor 6
Chain: N
Molecule details ›
Chain: N
Length: 941 amino acids
Theoretical weight: 107.09 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 941 amino acids
Theoretical weight: 107.09 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
O94906 (Residues: 1-941; Coverage: 100%)
Sequence domains:
U4/U6.U5 tri-snRNP-associated protein 1
Chain: S
Molecule details ›
Chain: S
Length: 800 amino acids
Theoretical weight: 90.41 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 800 amino acids
Theoretical weight: 90.41 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
O43290 (Residues: 1-800; Coverage: 100%)
Sequence domains:
U2 snRNA
Chain: 2
Molecule details ›
Chain: 2
Length: 188 nucleotides
Theoretical weight: 60.19 KDa
Sequence domains: U2 spliceosomal RNA
Length: 188 nucleotides
Theoretical weight: 60.19 KDa
Sequence domains: U2 spliceosomal RNA
U4 snRNA
Chain: 4
Molecule details ›
Chain: 4
Length: 144 nucleotides
Theoretical weight: 46.18 KDa
Sequence domains: U4 spliceosomal RNA
Length: 144 nucleotides
Theoretical weight: 46.18 KDa
Sequence domains: U4 spliceosomal RNA
U5 snRNA
Chain: 5
Molecule details ›
Chain: 5
Length: 117 nucleotides
Theoretical weight: 37.25 KDa
Sequence domains: U5 spliceosomal RNA
Length: 117 nucleotides
Theoretical weight: 37.25 KDa
Sequence domains: U5 spliceosomal RNA
U6 snRNA
Chain: 6
pre-mRNA
Chain: Z
Molecule details ›
Chain: Z
Length: 347 nucleotides
Theoretical weight: 111.3 KDa
Length: 347 nucleotides
Theoretical weight: 111.3 KDa
5'ss oligo
Chain: z
Molecule details ›
Chain: z
Length: 13 nucleotides
Theoretical weight: 4.26 KDa
Length: 13 nucleotides
Theoretical weight: 4.26 KDa