› 8r3k

Electron Microscopy
3.43Å resolution

Influenza A/H7N9 polymerase in self-stalled pre-termination state, with Pol II pS5 CTD peptide mimic bound in site 1A/2A.

Released:
DOI: 10.2210/pdb8r3k/pdb
PDB entry 8r3k coloured by chain, front view.
PDB entry 8r3k coloured by chain, side view.
PDB entry 8r3k coloured by chain, top view.
Source organisms:
Primary publication:
The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome.
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
OpenAccess logo Nat Commun 15 1064 (2024)
PMID: 38316757
Related structures: EMD-18871

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-271427 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 716 amino acids
Theoretical weight: 82.83 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: M9TI86 (Residues: 1-716; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 757 amino acids
Theoretical weight: 86.42 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: M9TLW3 (Residues: 1-757; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 788 amino acids
Theoretical weight: 89.04 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: X5F427 (Residues: 1-759; Coverage: 100%)
Gene name: PB2
Sequence domains:
DNA-directed RNA polymerase II subunit RPB1 Chain: G
Molecule details ›
Chain: G
Length: 42 amino acids
Theoretical weight: 4.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P24928 (Residues: 1699-1740; Coverage: 2%)
Gene names: POLR2, POLR2A
Sequence domains: RNA polymerase Rpb1 C-terminal repeat
51-mer vRNA loop (v51_mut_S) Chain: V
Molecule details ›
Chain: V
Length: 51 nucleotides
Theoretical weight: 16.09 KDa
RNA product Chain: M
Molecule details ›
Chain: M
Length: 34 nucleotides
Theoretical weight: 11.23 KDa

Ligands and Environments

2 bound ligands:
Ligand POP 1 x POP
Ligand MG 2 x MG
1 modified residue:
Ligand SEP 6 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 3.43Å
Relevant EMDB volumes: EMD-18871
Expression systems:
  • Trichoplusia ni
  • Not provided

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