› 8r4q

X-ray diffraction
2.84Å resolution

Salt inducible kinase 3 in complex with inhibitor

Released:
DOI: 10.2210/pdb8r4q/pdb
PDB entry 8r4q coloured by chain, front view.
PDB entry 8r4q coloured by chain, side view.
PDB entry 8r4q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structures of salt inducible kinase 3 in complex with pharmacological inhibitors reveal determinants for binding and selectivity.
Öster L, Castaldo M, de Vries E, Edfeldt F, Pemberton N, Gordon E, Cederblad L, Käck H
J Biol Chem 107201 (2024)
PMID: 38508313

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-278858 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase SIK3 Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 328 amino acids
Theoretical weight: 37.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y2K2 (Residues: 59-385; Coverage: 25%)
Gene names: KIAA0999, L19, QSK, SIK3
Sequence domains: Protein kinase domain
scFvH1 Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 265 amino acids
Theoretical weight: 27.41 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

2 bound ligands:
Ligand DB8 6 x DB8
Ligand SO4 9 x SO4
1 modified residue:
Ligand TPO 6 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P212121
Unit cell:
a: 85.116Å b: 213.702Å c: 223.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.244
Expression systems:
  • Spodoptera frugiperda
  • Cricetulus griseus

Quick links

Citations

1 mention without citation

The structures of salt-inducible kinase 3 in complex with inhibitors reveal determinants for binding and selectivity.
Öster et al. (2024)