› 8r4z

X-ray diffraction
1.54Å resolution

Crystal structure of alpha keto acid C-methyl-transferases MrsA native-form

Released:
DOI: 10.2210/pdb8r4z/pdb
PDB entry 8r4z coloured by chain, front view.
PDB entry 8r4z coloured by chain, side view.
PDB entry 8r4z coloured by chain, top view.
Source organism: Pseudomonas syringae
Primary publication:
Structures and protein engineering of the α-keto acid C-methyltransferases SgvM and MrsA for rational substrate transfer.
Sommer-Kamann C, Breiltgens J, Zou Z, Gerhardt S, Saleem-Batcha R, Kemper F, Einsle O, Andexer JN, Müller M
Chembiochem e202400258 (2024)
PMID: 38887142

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate = S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-ketoarginine methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 38.77 KDa
Source organism: Pseudomonas syringae
Expression system: Escherichia coli
UniProt:
  • Canonical: D5FKJ3 (Residues: 1-350; Coverage: 100%)
Gene name: mrsA

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 148.135Å b: 66.173Å c: 74.312Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.187
Expression system: Escherichia coli

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