› 8r8d

Electron Microscopy
2.6Å resolution

Cryo-EM structure of coagulation factor beta-XIIa in complex with the garadacimab Fab fragment (symmetric dimer)

Released:
DOI: 10.2210/pdb8r8d/pdb
PDB entry 8r8d coloured by chain, front view.
PDB entry 8r8d coloured by chain, side view.
PDB entry 8r8d coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Drulyte I
Related structures: EMD-18999

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236482 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Coagulation factor XIIa light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00748 (Residues: 373-615; Coverage: 41%)
Gene name: F12
Sequence domains: Trypsin
Garadacimab heavy chain variable region Chains: E, H
Molecule details ›
Chains: E, H
Length: 234 amino acids
Theoretical weight: 25.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Garadacimab light chain variable region Chains: I, L
Molecule details ›
Chains: I, L
Length: 215 amino acids
Theoretical weight: 22.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-18999
Expression system: Homo sapiens

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