› 8r8r

Electron Microscopy
2.79Å resolution

Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc)

Released:
DOI: 10.2210/pdb8r8r/pdb
PDB entry 8r8r coloured by chain, front view.
PDB entry 8r8r coloured by chain, side view.
PDB entry 8r8r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of human polyA polymerase recruitment by mPSF.
Todesca S, Sandmeir F, Keidel A, Conti E
RNA (2024)
PMID: 38538052
Related structures: EMD-19008

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-246311 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Cleavage and polyadenylation specificity factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1443 amino acids
Theoretical weight: 161.07 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q10570 (Residues: 1-1443; Coverage: 100%)
Gene names: CPSF1, CPSF160
Sequence domains:
pre-mRNA 3' end processing protein WDR33 Chain: B
Molecule details ›
Chain: B
Length: 413 amino acids
Theoretical weight: 47.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9C0J8 (Residues: 1-413; Coverage: 31%)
Gene names: WDC146, WDR33
Sequence domains: WD domain, G-beta repeat
Cleavage and polyadenylation specificity factor subunit 4 Chain: C
Molecule details ›
Chain: C
Length: 285 amino acids
Theoretical weight: 31.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O95639 (Residues: 2-269; Coverage: 90%)
  • Best match: O95639-2 (Residues: 2-244)
Gene names: CPSF30, CPSF4, NAR, NEB1
Sequence domains:
Poly(A) polymerase alpha Chain: D
Molecule details ›
Chain: D
Length: 26 amino acids
Theoretical weight: 2.87 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P51003 (Residues: 720-745; Coverage: 4%)
Gene names: PAP, PAPOLA
RNA (5'-R(P*AP*AP*UP*AP*AP*A)-3') Chain: E
Molecule details ›
Chain: E
Length: 6 nucleotides
Theoretical weight: 1.91 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.79Å
Relevant EMDB volumes: EMD-19008
Expression systems:
  • Homo sapiens
  • Not provided

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