8r8r

Electron Microscopy
2.79Å resolution

Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc)

Released:
Source organism: Homo sapiens
Related structures: EMD-19008

Function and Biology Details

Reaction catalysed:
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-271930 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Cleavage and polyadenylation specificity factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1443 amino acids
Theoretical weight: 161.07 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q10570 (Residues: 1-1443; Coverage: 100%)
Gene names: CPSF1, CPSF160
Sequence domains:
pre-mRNA 3' end processing protein WDR33 Chain: B
Molecule details ›
Chain: B
Length: 413 amino acids
Theoretical weight: 47.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9C0J8 (Residues: 1-413; Coverage: 31%)
Gene names: WDC146, WDR33
Sequence domains: WD domain, G-beta repeat
Cleavage and polyadenylation specificity factor subunit 4 Chain: C
Poly(A) polymerase alpha Chain: D
RNA (5'-R(P*AP*AP*UP*AP*AP*A)-3') Chain: E

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.79Å
Relevant EMDB volumes: EMD-19008
Expression systems:
  • Homo sapiens
  • Not provided