› 8rbf

Electron Microscopy
4.2Å resolution

CryoEM structure of the post-powerstroke actomyosin-5a complex

Released:
DOI: 10.2210/pdb8rbf/pdb
PDB entry 8rbf coloured by chain, front view.
PDB entry 8rbf coloured by chain, side view.
PDB entry 8rbf coloured by chain, top view.
Source organisms:
Entry authors: Klebl DP, McMillan SN, Risi C, Forgacs E, Virok B, Atherton JL, Stofella M, Winkelmann DA, Sobott F, Galkin VE, Knight PJ, Muench SP, Scarff CA, White HD
Related structures: EMD-19030

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-282279 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-Va Chain: A
Molecule details ›
Chain: A
Length: 759 amino acids
Theoretical weight: 87.34 KDa
Source organism: Mus musculus
Expression system: Santiria sp. sf9
UniProt:
  • Canonical: Q99104 (Residues: 5-767; Coverage: 41%)
Gene names: Dilute, Myo5a
Sequence domains: Myosin head (motor domain)
Actin, alpha skeletal muscle Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 376 amino acids
Theoretical weight: 41.89 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ADP 4 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-19030
Expression system: Santiria sp. sf9

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