› 8rd7

X-ray diffraction
1.31Å resolution

HUWE1 WWE domain in complex with ADP-ribose

Released:
DOI: 10.2210/pdb8rd7/pdb
PDB entry 8rd7 coloured by chain, front view.
PDB entry 8rd7 coloured by chain, side view.
PDB entry 8rd7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A ligand discovery toolbox for the WWE domain family of human E3 ligases.
Münzker L, Kimani SW, Fowkes MM, Dong A, Zheng H, Li Y, Dasovich M, Zak KM, Leung AKL, Elkins JM, Kessler D, Arrowsmith CH, Halabelian L, Böttcher J
Commun Biol 7 901 (2024)
PMID: 39048679

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HUWE1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 91 amino acids
Theoretical weight: 10.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6Z7 (Residues: 1611-1700; Coverage: 2%)
Gene names: HSPC272, HUWE1, KIAA0312, KIAA1578, UREB1
Sequence domains: WWE domain

Ligands and Environments

4 bound ligands:
Ligand CL 5 x CL
Ligand ACT 1 x ACT
Ligand GOL 1 x GOL
Ligand AR6 1 x AR6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P43212
Unit cell:
a: 62.883Å b: 62.883Å c: 231.245Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.177
Expression system: Escherichia coli

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