› 8rhf

X-ray diffraction
1.95Å resolution

Lytic Transglycosylase MltD of Pseudomonas aeruginosa bound to the Natural Product Bulgecin A, with two LysM domains

Released:
DOI: 10.2210/pdb8rhf/pdb
PDB entry 8rhf coloured by chain, front view.
PDB entry 8rhf coloured by chain, side view.
PDB entry 8rhf coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural characterization of lytic transglycosylase MltD of Pseudomonas aeruginosa, a target for the natural product bulgecin A.
Miguel-Ruano V, Feltzer R, Batuecas MT, Ramachandran B, El-Araby AM, Avila-Cobian LF, De Benedetti S, Mobashery S, Hermoso JA
Int J Biol Macromol 131420 (2024)
PMID: 38583835

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LysM domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 38.58 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I2T2 (Residues: 76-393; Coverage: 60%)
Gene names: PA1812, mltD
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand PEG 2 x PEG
Ligand BLG 2 x BLG
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 46.753Å b: 116.527Å c: 121.101Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.228
Expression system: Escherichia coli BL21(DE3)

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