› 8rhz

Electron Microscopy
3.37Å resolution

Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated conformation - symmetry expanded unneddylated dimer

Released:
DOI: 10.2210/pdb8rhz/pdb
PDB entry 8rhz coloured by chain, front view.
PDB entry 8rhz coloured by chain, side view.
PDB entry 8rhz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Noncanonical assembly, neddylation and chimeric cullin-RING/RBR ubiquitylation by the 1.8 MDa CUL9 E3 ligase complex.
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
OpenAccess logo Nat Struct Mol Biol (2024)
PMID: 38605244
Related structures: EMD-19179

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274101 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cullin-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 2519 amino acids
Theoretical weight: 281.69 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8IWT3 (Residues: 1-2517; Coverage: 100%)
Gene names: CUL9, H7AP1, KIAA0708, PARC
Sequence domains:
E3 ubiquitin-protein ligase RBX1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 108 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P62877 (Residues: 1-108; Coverage: 100%)
Gene names: RBX1, RNF75, ROC1
Sequence domains: RING-H2 zinc finger domain

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.37Å
Relevant EMDB volumes: EMD-19179
Expression system: Homo sapiens

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