› 8rkf

X-ray diffraction
3.2Å resolution

Crystal structure of the ZP-N1 and ZP-N2 domains of human ZP2 (hZP2-N1N2)

Released:
DOI: 10.2210/pdb8rkf/pdb
PDB entry 8rkf coloured by chain, front view.
PDB entry 8rkf coloured by chain, side view.
PDB entry 8rkf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat.
Nishio S, Emori C, Wiseman B, Fahrenkamp D, Dioguardi E, Zamora-Caballero S, Bokhove M, Han L, Stsiapanava A, Algarra B, Lu Y, Kodani M, Bainbridge RE, Komondor KM, Carlson AE, Landreh M, de Sanctis D, Yasumasu S, Ikawa M, Jovine L
Cell 187 1440-1459.e24 (2024)
PMID: 38490181

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Processed zona pellucida sperm-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05996 (Residues: 39-265; Coverage: 32%)
Gene names: ZP2, ZPA

Ligands and Environments

1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 86.93Å b: 86.93Å c: 178.68Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.234 0.293
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

ZP2: the precision regulator of egg coat architecture during fertilization.
Dong et al. (2024)