› 8rkh

X-ray diffraction
1.9Å resolution

Crystal structure of the ZP-N2 and ZP-N3 domains of mouse ZP2 (mZP2-N2N3)

Released:
DOI: 10.2210/pdb8rkh/pdb
PDB entry 8rkh coloured by chain, front view.
PDB entry 8rkh coloured by chain, side view.
PDB entry 8rkh coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat.
Nishio S, Emori C, Wiseman B, Fahrenkamp D, Dioguardi E, Zamora-Caballero S, Bokhove M, Han L, Stsiapanava A, Algarra B, Lu Y, Kodani M, Bainbridge RE, Komondor KM, Carlson AE, Landreh M, de Sanctis D, Yasumasu S, Ikawa M, Jovine L
Cell 187 1440-1459.e24 (2024)
PMID: 38490181

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-539410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Processed zona pellucida sperm-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 26.69 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P20239 (Residues: 135-360; Coverage: 33%)
Gene names: Zp-2, Zp2, Zpa

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 163.01Å b: 54.17Å c: 68.66Å
α: 90° β: 107.984° γ: 90°
R-values:
R R work R free
0.216 0.214 0.248
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

ZP2: the precision regulator of egg coat architecture during fertilization.
Dong et al. (2024)