› 8rkr

X-ray diffraction
1.2Å resolution

Structure of human DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE (ALDH4A1) complexed with a monophosphate-tweezer

Released:
DOI: 10.2210/pdb8rkr/pdb
PDB entry 8rkr coloured by chain, front view.
PDB entry 8rkr coloured by chain, side view.
PDB entry 8rkr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
How Do Molecular Tweezers Bind to Proteins? Lessons from X-ray Crystallography.
Porfetye AT, Stege P, Rebollido-Rios R, Hoffmann D, Schrader T, Vetter IR
OpenAccess logo Molecules 29 (2024)
PMID: 38675584

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 567 amino acids
Theoretical weight: 62.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30038 (Residues: 18-563; Coverage: 97%)
  • Best match: P30038-2 (Residues: 1-503)
Gene names: ALDH4, ALDH4A1, P5CDH
Sequence domains: Aldehyde dehydrogenase family

Ligands and Environments

2 bound ligands:
Ligand A1H1O 1 x A1H1O
Ligand BEZ 4 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 71.914Å b: 85.158Å c: 85.615Å
α: 90° β: 103.14° γ: 90°
R-values:
R R work R free
0.166 0.165 0.188
Expression system: Escherichia coli

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