› 8rku

Electron Microscopy
2.3Å resolution

Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K TnsC domain local-refinement map

Released:
DOI: 10.2210/pdb8rku/pdb
PDB entry 8rku coloured by chain, front view.
PDB entry 8rku coloured by chain, side view.
PDB entry 8rku coloured by chain, top view.
Source organism: Scytonema hofmannii
Primary publication:
Conformational landscape of the type V-K CRISPR-associated transposon integration assembly.
Tenjo-Castaño F, Sofos N, Stutzke LS, Temperini P, Fuglsang A, Pape T, Mesa P, Montoya G
Mol Cell (2024)
PMID: 38834066
Related structures: EMD-19283

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245969 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposase Chains: D, E, F, G, H, I, J, K, L, M, N, O, P, Q
Molecule details ›
Chains: D, E, F, G, H, I, J, K, L, M, N, O, P, Q
Length: 276 amino acids
Theoretical weight: 31.4 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A8J0PCL3 (Residues: 2-276; Coverage: 100%)
Sequence domains: Bacterial TniB protein
Non-target strand - LE Chain: 2
Molecule details ›
Chain: 2
Length: 68 nucleotides
Theoretical weight: 20.96 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided
Target strand - LE Chain: 3
Molecule details ›
Chain: 3
Length: 133 nucleotides
Theoretical weight: 40.82 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 14 x MG
Ligand ATP 14 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.3Å
Relevant EMDB volumes: EMD-19283
Expression systems:
  • Escherichia coli
  • Not provided

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