› 8rkv

Electron Microscopy
3.11Å resolution

Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K TnsB domain local-refinement map

Released:
DOI: 10.2210/pdb8rkv/pdb
PDB entry 8rkv coloured by chain, front view.
PDB entry 8rkv coloured by chain, side view.
PDB entry 8rkv coloured by chain, top view.
Source organism: Scytonema hofmannii
Primary publication:
Conformational landscape of the type V-K CRISPR-associated transposon integration assembly.
Tenjo-Castaño F, Sofos N, Stutzke LS, Temperini P, Fuglsang A, Pape T, Mesa P, Montoya G
Mol Cell (2024)
PMID: 38834066
Related structures: EMD-19284

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245970 (preferred)
Entry contents:
1 distinct polypeptide molecule
6 distinct DNA molecules
Macromolecules (7 distinct):
Integrase catalytic domain-containing protein Chains: R, S, T, U
Molecule details ›
Chains: R, S, T, U
Length: 584 amino acids
Theoretical weight: 66.59 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A979HMQ2 (Residues: 2-584; Coverage: 100%)
Sequence domains:
Non-target strand - LE Chain: 2
Molecule details ›
Chain: 2
Length: 68 nucleotides
Theoretical weight: 20.96 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided
Target strand - LE Chain: 3
Molecule details ›
Chain: 3
Length: 133 nucleotides
Theoretical weight: 40.82 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided
LE Chain: 4
Molecule details ›
Chain: 4
Length: 75 nucleotides
Theoretical weight: 23.24 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided
RE Chain: 5
Molecule details ›
Chain: 5
Length: 74 nucleotides
Theoretical weight: 22.88 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided
Non-target strand - RE Chain: 6
Molecule details ›
Chain: 6
Length: 79 nucleotides
Theoretical weight: 24.4 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided
Target strand Chain: 7
Molecule details ›
Chain: 7
Length: 15 nucleotides
Theoretical weight: 4.56 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.11Å
Relevant EMDB volumes: EMD-19284
Expression systems:
  • Escherichia coli
  • Not provided

Quick links