› 8rmc

Electron Microscopy
2.26Å resolution

Structure of the FDX2-bound core ISC complex (proximal conformation)

Released:
DOI: 10.2210/pdb8rmc/pdb
PDB entry 8rmc coloured by chain, front view.
PDB entry 8rmc coloured by chain, side view.
PDB entry 8rmc coloured by chain, top view.
Source organisms:
Primary publication:
Two-stage binding of mitochondrial ferredoxin-2 to the core iron-sulfur cluster assembly complex.
Steinhilper R, Boß L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun 15 10559 (2024)
PMID: 39632806
Related structures: EMD-19356

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-282805 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Cysteine desulfurase Chains: A, E
Molecule details ›
Chains: A, E
Length: 404 amino acids
Theoretical weight: 45.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y697 (Residues: 56-457; Coverage: 88%)
Gene names: HUSSY-08, NFS1, NIFS
Sequence domains: Aminotransferase class-V
LYR motif-containing protein 4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 115 amino acids
Theoretical weight: 13.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HD34 (Residues: 1-91; Coverage: 100%)
Gene names: C6orf149, CGI-203, ISD11, LYRM4
Sequence domains: Complex 1 protein (LYR family)
Iron-sulfur cluster assembly enzyme ISCU Chains: D, H
Ferredoxin-2, mitochondrial Chain: I
Acyl carrier protein Chains: C, G

Ligands and Environments

3 bound ligands:
Ligand FE2 2 x FE2
Ligand FES 1 x FES
Ligand 8Q1 2 x 8Q1
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
Resolution: 2.26Å
Relevant EMDB volumes: EMD-19356
Expression system: Escherichia coli BL21(DE3)

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