› 8rn7

Electron Microscopy
3.09Å resolution

Pseudo-symmetrical influenza B polymerase apo-dimer, core-only moeity (from "Influenza B polymerase pseudo-symmetrical dimer" | Local refinement)

Released:
DOI: 10.2210/pdb8rn7/pdb
PDB entry 8rn7 coloured by chain, front view.
PDB entry 8rn7 coloured by chain, side view.
PDB entry 8rn7 coloured by chain, top view.
Source organism: Influenza B virus (B/Memphis/13/2003)
Primary publication:
Structures of influenza A and B replication complexes give insight into avian to human host adaptation and reveal a role of ANP32 as an electrostatic chaperone for the apo-polymerase.
Arragain B, Krischuns T, Pelosse M, Drncova P, Blackledge M, Naffakh N, Cusack S
Nat Commun 15 6910 (2024)
PMID: 39160148
Related structures: EMD-19389

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-274807 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Polymerase acidic protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 726 amino acids
Theoretical weight: 83.15 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Z9 (Residues: 1-726; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 752 amino acids
Theoretical weight: 84.43 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Y6 (Residues: 1-752; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.09Å
Relevant EMDB volumes: EMD-19389
Expression system: Trichoplusia ni

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