› 8rnb

Electron Microscopy
3.31Å resolution

Influenza B polymerase, encapsidase plus 627(R) / human ANP32A (from "Influenza B polymerase apo-trimer" | Local refinement)

Released:
DOI: 10.2210/pdb8rnb/pdb
PDB entry 8rnb coloured by chain, front view.
PDB entry 8rnb coloured by chain, side view.
PDB entry 8rnb coloured by chain, top view.
Source organisms:
Primary publication:
Structures of influenza A and B replication complexes give insight into avian to human host adaptation and reveal a role of ANP32 as an electrostatic chaperone for the apo-polymerase.
Arragain B, Krischuns T, Pelosse M, Drncova P, Blackledge M, Naffakh N, Cusack S
Nat Commun 15 6910 (2024)
PMID: 39160148
Related structures: EMD-19393

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-273679 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Polymerase acidic protein Chain: D
Molecule details ›
Chain: D
Length: 726 amino acids
Theoretical weight: 83.15 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Z9 (Residues: 1-726; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: E
Molecule details ›
Chain: E
Length: 752 amino acids
Theoretical weight: 84.43 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Y6 (Residues: 1-752; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 799 amino acids
Theoretical weight: 90.96 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8X3 (Residues: 1-770; Coverage: 100%)
Gene name: PB2
Sequence domains:
Acidic leucine-rich nuclear phosphoprotein 32 family member A Chain: G
Molecule details ›
Chain: G
Length: 275 amino acids
Theoretical weight: 31.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39687 (Residues: 1-249; Coverage: 100%)
Gene names: ANP32A, C15orf1, LANP, MAPM, PHAP1
Sequence domains: Leucine-rich repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.31Å
Relevant EMDB volumes: EMD-19393
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

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