› 8ro6

X-ray diffraction
2.2Å resolution

Human cohesin SMC1A-HD(longCC)/RAD21-C complex - Apo closed P-loop conformation

Released:
DOI: 10.2210/pdb8ro6/pdb
PDB entry 8ro6 coloured by chain, front view.
PDB entry 8ro6 coloured by chain, side view.
PDB entry 8ro6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The cohesin ATPase cycle is mediated by specific conformational dynamics and interface plasticity of SMC1A and SMC3 ATPase domains.
Vitoria Gomes M, Landwerlin P, Diebold-Durand ML, Shaik TB, Durand A, Troesch E, Weber C, Brillet K, Lemée MV, Decroos C, Dulac L, Antony P, Watrin E, Ennifar E, Golzio C, Romier C
Cell Rep 43 114656 (2024)
PMID: 39240714

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-251266 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Structural maintenance of chromosomes protein 1A (SMC1A) Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 51.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
64-kDa C-terminal product Chain: B
Molecule details ›
Chain: B
Length: 81 amino acids
Theoretical weight: 9.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60216 (Residues: 558-629; Coverage: 11%)
Gene names: HR21, KIAA0078, NXP1, RAD21, SCC1
Sequence domains: Conserved region of Rad21 / Rec8 like protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 187.93Å b: 64.72Å c: 47.34Å
α: 90° β: 103.07° γ: 90°
R-values:
R R work R free
0.192 0.19 0.244
Expression system: Escherichia coli

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