› 8ro9

X-ray diffraction
1.77Å resolution

Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - Open/closed P-loop conformation

Released:
DOI: 10.2210/pdb8ro9/pdb
PDB entry 8ro9 coloured by chain, front view.
PDB entry 8ro9 coloured by chain, side view.
PDB entry 8ro9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The cohesin ATPase cycle is mediated by specific conformational dynamics and interface plasticity of SMC1A and SMC3 ATPase domains.
Vitoria Gomes M, Landwerlin P, Diebold-Durand ML, Shaik TB, Durand A, Troesch E, Weber C, Brillet K, Lemée MV, Decroos C, Dulac L, Antony P, Watrin E, Ennifar E, Golzio C, Romier C
Cell Rep 43 114656 (2024)
PMID: 39240714

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-251268 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Structural maintenance of chromosomes protein 1A Chains: A, C
Molecule details ›
Chains: A, C
Length: 456 amino acids
Theoretical weight: 51.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14683 (Residues: 1-200, 992-1233; Coverage: 36%)
Gene names: DXS423E, KIAA0178, SB1.8, SMC1, SMC1A, SMC1L1
Sequence domains: RecF/RecN/SMC N terminal domain
64-kDa C-terminal product Chains: B, D
Molecule details ›
Chains: B, D
Length: 81 amino acids
Theoretical weight: 9.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60216 (Residues: 558-629; Coverage: 11%)
Gene names: HR21, KIAA0078, NXP1, RAD21, SCC1
Sequence domains: Conserved region of Rad21 / Rec8 like protein

Ligands and Environments

1 bound ligand:
Ligand B3P 1 x B3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 187.918Å b: 64.597Å c: 89.949Å
α: 90° β: 99.74° γ: 90°
R-values:
R R work R free
0.191 0.19 0.218
Expression system: Escherichia coli

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