› 8rp5

X-ray diffraction
1.85Å resolution

Alpha-Methylacyl-CoA racemase from Mycobacterium tuberculosis (E241A mutant)

Released:
DOI: 10.2210/pdb8rp5/pdb
PDB entry 8rp5 coloured by chain, front view.
PDB entry 8rp5 coloured by chain, side view.
PDB entry 8rp5 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
α-Methylacyl-CoA Racemase from Mycobacterium tuberculosis-Detailed Kinetic and Structural Characterization of the Active Site.
Mojanaga OO, Woodman TJ, Lloyd MD, Acharya KR
OpenAccess logo Biomolecules 14 (2024)
PMID: 38540719

Function and Biology Details

Reaction catalysed: 
(2S)-2-methylacyl-CoA = (2R)-2-methylacyl-CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-methylacyl-CoA racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 39.1 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O06543 (Residues: 1-360; Coverage: 100%)
Gene names: Rv1143, mcr
Sequence domains: CoA-transferase family III

Ligands and Environments

2 bound ligands:
Ligand EDO 6 x EDO
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 180.619Å b: 78.989Å c: 58.923Å
α: 90° β: 92.191° γ: 90°
R-values:
R R work R free
0.195 0.193 0.236
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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