› 8rpj

X-ray diffraction
1.9Å resolution

JanthE from Janthinobacterium sp. HH01

Released:
DOI: 10.2210/pdb8rpj/pdb
PDB entry 8rpj coloured by chain, front view.
PDB entry 8rpj coloured by chain, side view.
PDB entry 8rpj coloured by chain, top view.
Source organism: Janthinobacterium sp. HH01
Primary publication:
Identification and Characterization of Thiamine Diphosphate-Dependent Lyases with an Unusual CDG Motif.
Lanza L, Rabe von Pappenheim F, Bjarnesen D, Leogrande C, Paul A, Krug L, Tittmann K, Müller M
Angew Chem Int Ed Engl e202404045 (2024)
PMID: 38874074

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245816 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine pyrophosphate-binding protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 619 amino acids
Theoretical weight: 68 KDa
Source organism: Janthinobacterium sp. HH01
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

8 bound ligands:
Ligand TPP 6 x TPP
Ligand FAD 6 x FAD
Ligand MG 6 x MG
Ligand 1PE 2 x 1PE
Ligand ACT 4 x ACT
Ligand GOL 1 x GOL
Ligand 2PE 1 x 2PE
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 185.196Å b: 105.169Å c: 193.605Å
α: 90° β: 96.51° γ: 90°
R-values:
R R work R free
0.155 0.153 0.186
Expression system: Escherichia coli BL21(DE3)

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