› 8rqf

Electron Microscopy
3.41Å resolution

Cryo-EM structure of human NTCP-Bulevirtide complex

Released:
DOI: 10.2210/pdb8rqf/pdb
PDB entry 8rqf coloured by chain, front view.
PDB entry 8rqf coloured by chain, side view.
PDB entry 8rqf coloured by chain, top view.
Source organisms:
Entry authors: Liu H, Zakrzewicz D, Nosol K, Irobalieva RN, Mukherjee S, Bang-Soerensen R, Goldmann N, Kunz S, Rossi L, Kossiakoff AA, Urban S, Glebe D, Geyer J, Locher KP
Related structures: EMD-19440

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-245866 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Hepatic sodium/bile acid cotransporter Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 38.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q14973 (Residues: 1-349; Coverage: 100%)
Gene names: GIG29, NTCP, SLC10A1
Sequence domains: Sodium Bile acid symporter family
Heavy chain of Fab3 Chain: H
Molecule details ›
Chain: H
Length: 235 amino acids
Theoretical weight: 25.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Light chain of Fab3 Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab-specific nanobody Chain: K
Molecule details ›
Chain: K
Length: 120 amino acids
Theoretical weight: 13.12 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Large envelope protein Chain: B
Molecule details ›
Chain: B
Length: 46 amino acids
Theoretical weight: 5.14 KDa
Source organism: hepatitis B virus genotype C
Expression system: Not provided
UniProt:
  • Canonical: Q76R62 (Residues: 14-59; Coverage: 12%)
Gene name: S

Ligands and Environments

1 bound ligand:
Ligand BJU 1 x BJU
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.41Å
Relevant EMDB volumes: EMD-19440
Expression systems:
  • Homo sapiens
  • Escherichia coli
  • Not provided

Quick links