› 8rrs

Electron Microscopy
3.4Å resolution

Structure of mouse RyR2 solubilised in detergent in open state in complex with Ca2+, ATP, caffeine and Nb9657.

Released:
DOI: 10.2210/pdb8rrs/pdb
PDB entry 8rrs coloured by chain, front view.
PDB entry 8rrs coloured by chain, side view.
PDB entry 8rrs coloured by chain, top view.
Source organisms:
Primary publication:
Rapid small-scale nanobody-assisted purification of ryanodine receptors for cryo-EM.
Li C, Willegems K, Uchański T, Pardon E, Steyaert J, Efremov RG
J Biol Chem 107734 (2024)
PMID: 39233227
Related structures: EMD-19463

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279251 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ryanodine receptor 2 Chains: A, C, E, F
Molecule details ›
Chains: A, C, E, F
Length: 4966 amino acids
Theoretical weight: 565.54 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: E9Q401 (Residues: 1-4966; Coverage: 100%)
Gene name: Ryr2
Sequence domains:
Nanobody 9657 Chains: B, D, G, I
Molecule details ›
Chains: B, D, G, I
Length: 137 amino acids
Theoretical weight: 15.13 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand ATP 4 x ATP
Ligand CFF 4 x CFF
Ligand ZN 4 x ZN
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-19463
Expression system: Escherichia coli

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