8rt7

Electron Microscopy
2.93Å resolution

Conformation-B of the full-length outer membrane core complex (TrwH/VirB7, TrwF/VirB9, TrwE/VirB10CTD) from the fully-assembled R388 type IV secretion system determined by cryo-EM.

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 46-mer (preferred)
PDBe Complex ID:
PDB-CPX-245975 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
TrwE protein Chains: A, D, G, J, M, P, S, V, Y, b, e, h, k, n, u, y
Molecule details ›
Chains: A, D, G, J, M, P, S, V, Y, b, e, h, k, n, u, y
Length: 395 amino acids
Theoretical weight: 42.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A8R758 (Residues: 1-395; Coverage: 100%)
Gene name: trwE
Sequence domains: Bacterial conjugation TrbI-like protein
TrwF protein Chains: B, E, H, K, N, Q, T, W, Z, c, f, i, l, o, v, z
Molecule details ›
Chains: B, E, H, K, N, Q, T, W, Z, c, f, i, l, o, v, z
Length: 266 amino acids
Theoretical weight: 29.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A8R757 (Residues: 1-266; Coverage: 100%)
Gene name: trwF
Sequence domains: Conjugal transfer protein
TrwH Chains: C, F, I, L, O, R, U, X, a, d, g, j, m, p
Molecule details ›
Chains: C, F, I, L, O, R, U, X, a, d, g, j, m, p
Length: 47 amino acids
Theoretical weight: 5.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A7KZV0 (Residues: 1-47; Coverage: 100%)
Gene name: trwH

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-19481
Expression system: Escherichia coli