› 8ryp

X-ray diffraction
1.81Å resolution

Structure of S8 TCR in complex with HLA-A*03:01 bound to ELFSYLIEK peptide

Released:
DOI: 10.2210/pdb8ryp/pdb
PDB entry 8ryp coloured by chain, front view.
PDB entry 8ryp coloured by chain, side view.
PDB entry 8ryp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Broadening alloselectivity of T cell receptors by structure guided engineering.
Karuppiah V, Sangani D, Whaley L, Pengelly R, Uluocak P, Carreira RJ, Hock M, Cristina PD, Bartasun P, Dobrinic P, Smith N, Barnbrook K, Robinson RA, Harper S
Sci Rep 14 26851 (2024)
PMID: 39500929

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-285957 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
ELFSYLIEK peptide Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
TCR alpha Chain: D
Molecule details ›
Chain: D
Length: 200 amino acids
Theoretical weight: 21.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
TCR beta Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 208.12Å b: 45.71Å c: 121.68Å
α: 90° β: 94.93° γ: 90°
R-values:
R R work R free
0.222 0.221 0.241
Expression systems:
  • Escherichia coli
  • Not provided

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