› 8s0l

X-ray diffraction
1.8Å resolution

Crystal structure of the TMPRSS2 zymogen in complex with the nanobody A07

Released:
DOI: 10.2210/pdb8s0l/pdb
PDB entry 8s0l coloured by chain, front view.
PDB entry 8s0l coloured by chain, side view.
PDB entry 8s0l coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of TMPRSS2 zymogen activation and recognition by the HKU1 seasonal coronavirus.
Fernández I, Saunders N, Duquerroy S, Bolland WH, Arbabian A, Baquero E, Blanc C, Lafaye P, Haouz A, Buchrieser J, Schwartz O, Rey FA
Cell 187 4246-4260.e16 (2024)
PMID: 38964326

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246297 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transmembrane protease serine 2 non-catalytic chain Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 43.68 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: O15393 (Residues: 107-492; Coverage: 79%)
Gene names: PRSS10, TMPRSS2
Sequence domains:
Nanobody A07 Chain: B
Molecule details ›
Chain: B
Length: 150 amino acids
Theoretical weight: 16.03 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 182.665Å b: 53.808Å c: 65.477Å
α: 90° β: 100.74° γ: 90°
R-values:
R R work R free
0.179 0.177 0.215
Expression systems:
  • Drosophila melanogaster
  • Escherichia coli

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