› 8s5u

Electron Microscopy
3.28Å resolution

Cryo-EM structure of thiamine-bound human SLC19A3 in outward-open state

Released:
DOI: 10.2210/pdb8s5u/pdb
PDB entry 8s5u coloured by chain, front view.
PDB entry 8s5u coloured by chain, side view.
PDB entry 8s5u coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of thiamine transport and drug recognition by SLC19A3.
Gabriel F, Spriestersbach L, Fuhrmann A, Jungnickel KEJ, Mostafavi S, Pardon E, Steyaert J, Löw C
Nat Commun 15 8542 (2024)
PMID: 39358356
Related structures: EMD-19750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280315 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nb3.4 Chain: B
Molecule details ›
Chain: B
Length: 138 amino acids
Theoretical weight: 15.22 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Thiamine transporter 2 Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 59.69 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BZV2 (Residues: 1-496; Coverage: 100%)
Gene name: SLC19A3
Sequence domains: Reduced folate carrier

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand VIB 1 x VIB
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.28Å
Relevant EMDB volumes: EMD-19750
Expression systems:
  • Escherichia coli
  • Homo sapiens

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