› 8s70

Electron Microscopy
4.2Å resolution

Cryo-EM structure of Pseudomonas aeruginosa recombinase A (RecA) in complex with ssDNA 72mer and ATPgS

Released:
DOI: 10.2210/pdb8s70/pdb
PDB entry 8s70 coloured by chain, front view.
PDB entry 8s70 coloured by chain, side view.
PDB entry 8s70 coloured by chain, top view.
Source organisms:
Primary publication:
Snapshots of Pseudomonas aeruginosa SOS response reveal structural requisites for LexA autoproteolysis.
Vascon F, De Felice S, Gasparotto M, Huber ST, Catalano C, Chinellato M, Mezzetti R, Grinzato A, Filippini F, Maso L, Jakobi AJ, Cendron L
iScience 28 111726 (2025)
PMID: 39898034
Related structures: EMD-19761

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 32-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-285844 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein RecA Chain: D
Molecule details ›
Chain: D
Length: 328 amino acids
Theoretical weight: 35.06 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P08280 (Residues: 2-328; Coverage: 95%)
Gene names: PA3617, recA
Sequence domains:
DNA Chain: T
Molecule details ›
Chain: T
Length: 4 nucleotides
Theoretical weight: 1.17 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand AGS 1 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-19761
Expression systems:
  • Escherichia coli
  • Not provided

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