› 8s95

X-ray diffraction
3.1Å resolution

Crystal Structure of Poliovirus (type 1 Mahoney) cloverleaf RNA with tRNA scaffold

Released:
DOI: 10.2210/pdb8s95/pdb
PDB entry 8s95 coloured by chain, front view.
PDB entry 8s95 coloured by chain, side view.
PDB entry 8s95 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for cloverleaf RNA-initiated viral genome replication.
Gottipati K, McNeme SC, Tipo J, White MA, Choi KH
OpenAccess logo Nucleic Acids Res (2023)
PMID: 37486760

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224539 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Lysine tRNA scaffold,Poliovirus cloverleaf RNA Chain: C
Molecule details ›
Chain: C
Length: 157 nucleotides
Theoretical weight: 50.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Sequence domains:
  • tRNA
  • Enterovirus 5' cloverleaf cis-acting replication element

    • Ligands and Environments

    No bound ligands
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    X-ray source: RIGAKU FR-E+ DW
    Spacegroup: C2
    Unit cell:
    a: 150.021Å b: 28.015Å c: 111.267Å
    α: 90° β: 98.039° γ: 90°
    R-values:
    R R work R free
    0.276 0.275 0.287
    Expression system: Escherichia coli BL21(DE3)

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