› 8s9d

X-ray diffraction
2.57Å resolution

C143S variant of Citrate Synthase (CitA) in Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb8s9d/pdb
PDB entry 8s9d coloured by chain, front view.
PDB entry 8s9d coloured by chain, side view.
PDB entry 8s9d coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Mycobacterium tuberculosis CitA activity is modulated by cysteine oxidation and pyruvate binding.
Pathirage R, Favrot L, Petit C, Yamsek M, Singh S, Mallareddy JR, Rana S, Natarajan A, Ronning DR
RSC Med Chem 14 921-933 (2023)
PMID: 37252106

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-552395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative citrate synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 41 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPD3 (Residues: 1-373; Coverage: 100%)
Gene names: MTCY31.17c, Rv0889c, citA
Sequence domains: Citrate synthase, C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand EDO 5 x EDO
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I4122
Unit cell:
a: 150.253Å b: 150.253Å c: 231.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.316 0.313 0.329
Expression system: Escherichia coli

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