› 8s9o

X-ray diffraction
1.94Å resolution

DNA cytosine-N4 methyltransferase (residues 61-324) from the Bdelloid rotifer Adineta vaga - P1 crystal form

Released:
DOI: 10.2210/pdb8s9o/pdb
PDB entry 8s9o coloured by chain, front view.
PDB entry 8s9o coloured by chain, side view.
PDB entry 8s9o coloured by chain, top view.
Source organism: Adineta vaga
Primary publication:
Biochemical and structural characterization of the first-discovered metazoan DNA cytosine-N4 methyltransferase from the Bdelloid rotifer Adineta vaga.
Zhou J, Horton JR, Kaur G, Chen Q, Li X, Mendoza F, Wu T, Blumenthal RM, Zhang X, Cheng X
J Biol Chem 105017 (2023)
PMID: 37414145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA cytosine-N4 methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 31.92 KDa
Source organism: Adineta vaga
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand SFG 2 x SFG
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P1
Unit cell:
a: 49.646Å b: 53.301Å c: 54.376Å
α: 97.04° β: 104.34° γ: 98.2°
R-values:
R R work R free
0.208 0.206 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links