› 8sbv

X-ray diffraction
1.55Å resolution

Crystal Structure of 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase from Klebsiella aerogenes (ADP bound)

Released:
DOI: 10.2210/pdb8sbv/pdb
PDB entry 8sbv coloured by chain, front view.
PDB entry 8sbv coloured by chain, side view.
PDB entry 8sbv coloured by chain, top view.
Source organism: Klebsiella aerogenes KCTC 2190
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
2,3-dihydro-2,3-dihydroxybenzoate + NAD(+) = 2,3-dihydroxybenzoate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 27.57 KDa
Source organism: Klebsiella aerogenes KCTC 2190
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3FXS4 (Residues: 1-251; Coverage: 100%)
Gene name: EAE_13665
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand SO4 3 x SO4
Ligand ADP 1 x ADP
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 67.52Å b: 111.5Å c: 58.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links