› 8sf4

X-ray diffraction
1.46Å resolution

Cystathionine gamma lyase from Thermobifida fusca in internal aldimine form

Released:
DOI: 10.2210/pdb8sf4/pdb
PDB entry 8sf4 coloured by chain, front view.
PDB entry 8sf4 coloured by chain, side view.
PDB entry 8sf4 coloured by chain, top view.
Source organism: Thermobifida fusca
Entry authors: Perkins LJ, Buller AR, Bindman CA

Function and Biology Details

Reaction catalysed: 
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine gamma-lyase Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 41.78 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47ST9 (Residues: 1-381; Coverage: 100%)
Gene name: Tfu_0440
Sequence domains: Cys/Met metabolism PLP-dependent enzyme

Ligands and Environments

3 bound ligands:
Ligand PEG 1 x PEG
Ligand 1PE 1 x 1PE
Ligand MG 1 x MG
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P23
Unit cell:
a: 117.591Å b: 117.591Å c: 117.591Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.133 0.148
Expression system: Escherichia coli BL21(DE3)

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