› 8sf7

Electron Microscopy
4.1Å resolution

48-nm doublet microtubule from Tetrahymena thermophila strain MEC17

Released:
DOI: 10.2210/pdb8sf7/pdb
PDB entry 8sf7 coloured by chain, front view.
PDB entry 8sf7 coloured by chain, side view.
PDB entry 8sf7 coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Effect of α-tubulin acetylation on the doublet microtubule structure.
Yang SK, Kubo S, Black CS, Peri K, Dai D, Legal T, Valente-Paterno M, Gaertig J, Bui KH
OpenAccess logo Elife 12 (2024)
PMID: 38598282
Related structures: EMD-40436

Function and Biology Details

Reaction catalysed: 
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 431-mer (preferred)
PDBe Complex ID:
PDB-CPX-275756 (preferred)
Entry contents:
45 distinct polypeptide molecules
Macromolecules (45 distinct):
Nebulin Chain: 6H
Molecule details ›
Chain: 6H
Length: 518 amino acids
Theoretical weight: 61.03 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q231B6 (Residues: 1-518; Coverage: 100%)
Gene name: TTHERM_00433410
RIB27A Chains: 0A, 1A, 2A, 3A
Molecule details ›
Chains: 0A, 1A, 2A, 3A
Length: 236 amino acids
Theoretical weight: 27.61 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7LUL4 (Residues: 1-236; Coverage: 100%)
Gene name: TTHERM_00522810
STOP protein Chain: 0B
Molecule details ›
Chain: 0B
Length: 329 amino acids
Theoretical weight: 38.18 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23JL9 (Residues: 1-329; Coverage: 100%)
Gene name: TTHERM_00758860
Ciliary microtubule inner protein 2A-C-like domain-containing protein Chains: 0C, 1C
Molecule details ›
Chains: 0C, 1C
Length: 156 amino acids
Theoretical weight: 17.71 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q235M9 (Residues: 1-156; Coverage: 100%)
Gene name: TTHERM_01052990
Sequence domains: Ciliary microtubule inner protein 2B-like
Ciliary microtubule inner protein 2A-C-like domain-containing protein Chains: 0D, 1D, 2D
Molecule details ›
Chains: 0D, 1D, 2D
Length: 225 amino acids
Theoretical weight: 25.78 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q238X3 (Residues: 1-225; Coverage: 100%)
Gene name: TTHERM_00449470
Sequence domains: Ciliary microtubule inner protein 2B-like
EF-hand domain-containing protein Chains: 0E, 1E, 2E, 3E
Molecule details ›
Chains: 0E, 1E, 2E, 3E
Length: 191 amino acids
Theoretical weight: 22.4 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7LT67 (Residues: 1-191; Coverage: 100%)
Gene name: TTHERM_00691900
Sequence domains: EF-hand domain pair
CFAM166C Chain: 0F
Molecule details ›
Chain: 0F
Length: 219 amino acids
Theoretical weight: 25.07 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22B75 (Residues: 1-219; Coverage: 100%)
Gene name: TTHERM_01109800
CFAP107 Chains: 0G, 1G
Molecule details ›
Chains: 0G, 1G
Length: 183 amino acids
Theoretical weight: 21.31 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q237T1 (Residues: 1-183; Coverage: 100%)
Gene name: TTHERM_00320040
Meiosis-specific nuclear structural protein 1 Chains: 0H, 1H
Molecule details ›
Chains: 0H, 1H
Length: 447 amino acids
Theoretical weight: 55.56 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7LV70 (Residues: 1-447; Coverage: 100%)
Gene name: TTHERM_00338110
Sequence domains: Trichohyalin-plectin-homology domain
EF hand protein, putative Chains: 0N, 1N, 2N
Molecule details ›
Chains: 0N, 1N, 2N
Length: 492 amino acids
Theoretical weight: 57.49 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22WJ6 (Residues: 1-492; Coverage: 100%)
Gene name: TTHERM_00155380
CFA20 domain-containing protein Chains: 0Q, 1Q, 2Q, 3Q, 4Q, 5Q, 6Q
Molecule details ›
Chains: 0Q, 1Q, 2Q, 3Q, 4Q, 5Q, 6Q
Length: 195 amino acids
Theoretical weight: 22.98 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22NU3 (Residues: 1-195; Coverage: 100%)
Gene name: TTHERM_00418580
Sequence domains: CFA20 domain
Parkin co-regulated protein Chains: 0S, 1S, 2S, 3S
Molecule details ›
Chains: 0S, 1S, 2S, 3S
Length: 319 amino acids
Theoretical weight: 36.3 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MLV6 (Residues: 1-319; Coverage: 100%)
Gene name: TTHERM_00446290
Sequence domains: Parkin co-regulated protein
IJ34 Chains: 0T, 1T, 2T, 3T
Molecule details ›
Chains: 0T, 1T, 2T, 3T
Length: 298 amino acids
Theoretical weight: 34.43 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7M9T0 (Residues: 1-298; Coverage: 100%)
Gene name: TTHERM_00348390
Sequence domains:
Cilia- and flagella-associated protein 52 Chains: 0U, 1U, 2U, 3U
Molecule details ›
Chains: 0U, 1U, 2U, 3U
Length: 656 amino acids
Theoretical weight: 73.43 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22ZH2 (Residues: 1-656; Coverage: 100%)
Gene name: TTHERM_01094880
Sequence domains: WD domain, G-beta repeat
Enkurin domain-containing protein Chains: 0V, 1V, 2V, 3V
Molecule details ›
Chains: 0V, 1V, 2V, 3V
Length: 269 amino acids
Theoretical weight: 31.49 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7M279 (Residues: 1-269; Coverage: 100%)
Gene name: TTHERM_00137550
Sequence domains: Calmodulin-binding
RIB43A protein Chains: 0X, 1X, 2X, 3X, 4X
Molecule details ›
Chains: 0X, 1X, 2X, 3X, 4X
Length: 142 amino acids
Theoretical weight: 17.32 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: A4VDZ5 (Residues: 1-142; Coverage: 100%)
Gene name: TTHERM_00641119
Sequence domains: RIB43A
EF-hand domain-containing protein Chains: 1B, 2B, 3B
Molecule details ›
Chains: 1B, 2B, 3B
Length: 498 amino acids
Theoretical weight: 57.58 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7ME23 (Residues: 1-498; Coverage: 100%)
Gene name: TTHERM_00406590
Sequence domains:
CFAP182A Chain: 1F
Molecule details ›
Chain: 1F
Length: 173 amino acids
Theoretical weight: 20.11 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q24BV4 (Residues: 1-173; Coverage: 100%)
Gene name: TTHERM_01049330
Sequence domains: Piercer of microtubule wall 1/2
CFAP143 Chain: 1I
Molecule details ›
Chain: 1I
Length: 263 amino acids
Theoretical weight: 30.49 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: A4VD56 (Residues: 1-263; Coverage: 100%)
Gene name: TTHERM_00502359
CFAP21A Chain: 1J
Molecule details ›
Chain: 1J
Length: 422 amino acids
Theoretical weight: 48.5 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MLS4 (Residues: 1-422; Coverage: 100%)
Gene name: TTHERM_00494280
Cilia- and flagella-associated protein 53 Chains: 1K, 2K
Molecule details ›
Chains: 1K, 2K
Length: 489 amino acids
Theoretical weight: 60.72 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23YQ8 (Residues: 1-489; Coverage: 100%)
Gene name: TTHERM_01207630
Sequence domains: Trichohyalin-plectin-homology domain
EF-hand domain-containing protein Chains: 1L, 2L, 3L
Molecule details ›
Chains: 1L, 2L, 3L
Length: 940 amino acids
Theoretical weight: 110.66 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23KF9 (Residues: 1-940; Coverage: 100%)
Gene name: TTHERM_00193760
Nucleoside diphosphate kinase Chains: 1M, 2M
Molecule details ›
Chains: 1M, 2M
Length: 372 amino acids
Theoretical weight: 42.64 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: W7XGD1 (Residues: 1-372; Coverage: 100%)
Gene name: TTHERM_000372529
Sequence domains: Nucleoside diphosphate kinase
Cilia- and flagella-associated protein 45 Chains: 1O, 2O, 3O
Molecule details ›
Chains: 1O, 2O, 3O
Length: 494 amino acids
Theoretical weight: 59.58 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: W7XCX2 (Residues: 1-494; Coverage: 100%)
Gene name: TTHERM_001164064
Sequence domains: Trichohyalin-plectin-homology domain
Trichohyalin-plectin-homology domain-containing protein Chains: 1P, 2P
Molecule details ›
Chains: 1P, 2P
Length: 507 amino acids
Theoretical weight: 61.71 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23EX8 (Residues: 1-507; Coverage: 100%)
Gene name: TTHERM_00643530
Sequence domains: Trichohyalin-plectin-homology domain
DM10 domain-containing protein Chains: 1R, 2R, 3R
Molecule details ›
Chains: 1R, 2R, 3R
Length: 516 amino acids
Theoretical weight: 60.97 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MCU1 (Residues: 1-516; Coverage: 100%)
Gene name: TTHERM_00584850
Sequence domains: DM10 domain
Protofilament ribbon protein Chains: 1W, 2W
Molecule details ›
Chains: 1W, 2W
Length: 280 amino acids
Theoretical weight: 34.22 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q240R7 (Residues: 1-280; Coverage: 100%)
Gene name: TTHERM_00624660
Sequence domains: RIB43A
EF-hand domain-containing protein Chains: 2C, 3C, 4C
Molecule details ›
Chains: 2C, 3C, 4C
Length: 300 amino acids
Theoretical weight: 34.95 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7ME81 (Residues: 1-300; Coverage: 100%)
Gene name: TTHERM_00267880
Sequence domains: EF-hand domain pair
CFAP182B Chain: 2F
Molecule details ›
Chain: 2F
Length: 96 amino acids
Theoretical weight: 11.7 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MLW3 (Residues: 1-96; Coverage: 100%)
Gene name: TTHERM_00535210
Sequence domains: Piercer of microtubule wall 1/2
Flagellar FliJ protein Chain: 2G
Molecule details ›
Chain: 2G
Length: 99 amino acids
Theoretical weight: 12.1 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: A4VCU8 (Residues: 1-99; Coverage: 100%)
Gene name: TTHERM_00242188
Sequence domains: Cilia- and flagella-associated protein 141
RIB27B Chains: 2H, 3H, 4H
Molecule details ›
Chains: 2H, 3H, 4H
Length: 229 amino acids
Theoretical weight: 26.27 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22CT6 (Residues: 1-229; Coverage: 100%)
Gene name: TTHERM_01016050
Sperm-tail PG-rich repeat protein Chains: 2I, 3I
Molecule details ›
Chains: 2I, 3I
Length: 293 amino acids
Theoretical weight: 33.65 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7M2G0 (Residues: 1-293; Coverage: 100%)
Gene name: TTHERM_00218910
Sequence domains: Sperm-tail PG-rich repeat
Dienelactone hydrolase domain-containing protein Chain: 3D
Molecule details ›
Chain: 3D
Length: 237 amino acids
Theoretical weight: 26.11 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q232I6 (Residues: 1-237; Coverage: 100%)
Gene name: TTHERM_00594110
Sequence domains: Dienelactone hydrolase family
Ptype ATPase, putative Chain: 4F
Molecule details ›
Chain: 4F
Length: 276 amino acids
Theoretical weight: 31.4 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7M9I4 (Residues: 1-276; Coverage: 100%)
Gene name: TTHERM_00565590
Flagellar microtugule protofilament ribbon protein Chains: 4R, 5R, 6R, 7R
Molecule details ›
Chains: 4R, 5R, 6R, 7R
Length: 613 amino acids
Theoretical weight: 72.64 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7M0S7 (Residues: 1-613; Coverage: 100%)
Gene name: TTHERM_00143690
Sequence domains: DM10 domain
Parkin co-regulated protein Chains: 4S, 5S
Molecule details ›
Chains: 4S, 5S
Length: 249 amino acids
Theoretical weight: 28.41 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7M317 (Residues: 1-249; Coverage: 100%)
Gene name: TTHERM_00499570
Sequence domains: Parkin co-regulated protein
OJ2 Chains: 5A, 5B, 5C, 5D
Molecule details ›
Chains: 5A, 5B, 5C, 5D
Length: 175 amino acids
Theoretical weight: 19.93 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q236L2 (Residues: 1-175; Coverage: 100%)
Gene name: TTHERM_00086850
Sperm-tail PG-rich repeat protein Chains: 5E, 5F, 5G, 5H
Molecule details ›
Chains: 5E, 5F, 5G, 5H
Length: 247 amino acids
Theoretical weight: 28.99 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22WR6 (Residues: 1-247; Coverage: 100%)
Gene name: TTHERM_00974270
Sequence domains: Cilia- and flagella-associated protein 77
OJ3 Chains: 5I, 5J, 5K
Molecule details ›
Chains: 5I, 5J, 5K
Length: 168 amino acids
Theoretical weight: 19.6 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MFP6 (Residues: 1-168; Coverage: 100%)
Gene name: TTHERM_00220800
Sequence domains: FAM183A and FAM183B related
SB1 Chain: 6F
Molecule details ›
Chain: 6F
Length: 145 amino acids
Theoretical weight: 17.79 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q231B2 (Residues: 1-145; Coverage: 100%)
Gene name: TTHERM_00433450
Sperm-tail PG-rich repeat protein Chain: 6G
Molecule details ›
Chain: 6G
Length: 364 amino acids
Theoretical weight: 42.13 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q24GM1 (Residues: 1-364; Coverage: 100%)
Gene name: TTHERM_00724700
Sequence domains: Sperm-tail PG-rich repeat
CFAP112B Chains: 8L, 8N
Molecule details ›
Chains: 8L, 8N
Length: 385 amino acids
Theoretical weight: 32.78 KDa
Source organism: Tetrahymena thermophila
CFAP112A Chains: 8P, 8R
Molecule details ›
Chains: 8P, 8R
Length: 363 amino acids
Theoretical weight: 30.91 KDa
Source organism: Tetrahymena thermophila
Tubulin alpha chain Chains: AA, AC, AE, AG, AI, AK, AM, BA, BC, BE, BG, BI, BK, BM, CA, CC, CE, CG, CI, CK, CM, DA, DC, DE, DG, DI, DK, DM, EA, EC, EE, EG, EI, EK, EM, FA, FC, FE, FG, FI, FK, FM, GA, GC, GE, GG, GI, GK, GM, HA, HC, HE, HG, HI, HK, HM, IA, IC, IE, IG, II, IK, IM, JA, JC, JE, JG, JI, JK, JM, KA, KC, KE, KG, KI, KK, KM, LA, LC, LE, LG, LI, LK, LM, MA, MC, ME, MG, MI, MK, MM, NA, NC, NE, NG, NI, NK, NM, OA, OC, OE, OG, OI, OK, OM, PA, PC, PE, PG, PI, PK, PM, QA, QC, QE, QG, QI, QK, QM, RA, RC, RE, RG, RI, RK, RM, SA, SC, SE, SG, SI, SK, SM, TA, TC, TE, TG, TI, TK, TM, UA, UC, UE, UG, UI, UK, UM, VA, VC, VE, VG, VI, VK, VM, WA, WC, WE, WG, WI, WK, WM
Molecule details ›
Chains: AA, AC, AE, AG, AI, AK, AM, BA, BC, BE, BG, BI, BK, BM, CA, CC, CE, CG, CI, CK, CM, DA, DC, DE, DG, DI, DK, DM, EA, EC, EE, EG, EI, EK, EM, FA, FC, FE, FG, FI, FK, FM, GA, GC, GE, GG, GI, GK, GM, HA, HC, HE, HG, HI, HK, HM, IA, IC, IE, IG, II, IK, IM, JA, JC, JE, JG, JI, JK, JM, KA, KC, KE, KG, KI, KK, KM, LA, LC, LE, LG, LI, LK, LM, MA, MC, ME, MG, MI, MK, MM, NA, NC, NE, NG, NI, NK, NM, OA, OC, OE, OG, OI, OK, OM, PA, PC, PE, PG, PI, PK, PM, QA, QC, QE, QG, QI, QK, QM, RA, RC, RE, RG, RI, RK, RM, SA, SC, SE, SG, SI, SK, SM, TA, TC, TE, TG, TI, TK, TM, UA, UC, UE, UG, UI, UK, UM, VA, VC, VE, VG, VI, VK, VM, WA, WC, WE, WG, WI, WK, WM
Length: 449 amino acids
Theoretical weight: 49.64 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: P41351 (Residues: 1-449; Coverage: 100%)
Sequence domains:
Tubulin beta chain Chains: AB, AD, AF, AH, AJ, AL, AN, BB, BD, BF, BH, BJ, BL, BN, CB, CD, CF, CH, CJ, CL, CN, DB, DD, DF, DH, DJ, DL, DN, EB, ED, EF, EH, EJ, EL, EN, FB, FD, FF, FH, FJ, FL, FN, GB, GD, GF, GH, GJ, GL, GN, HB, HD, HF, HH, HJ, HL, HN, IB, ID, IF, IH, IJ, IL, IN, JB, JD, JF, JH, JJ, JL, JN, KB, KD, KF, KH, KJ, KL, KN, LB, LD, LF, LH, LJ, LL, LN, MB, MD, MF, MH, MJ, ML, MN, NB, ND, NF, NH, NJ, NL, NN, OB, OD, OF, OH, OJ, OL, ON, PB, PD, PF, PH, PJ, PL, PN, QB, QD, QF, QH, QJ, QL, QN, RB, RD, RF, RH, RJ, RL, RN, SB, SD, SF, SH, SJ, SL, SN, TB, TD, TF, TH, TJ, TL, TN, UB, UD, UF, UH, UJ, UL, UN, VB, VD, VF, VH, VJ, VL, VN, WB, WD, WF, WH, WJ, WL, WN
Molecule details ›
Chains: AB, AD, AF, AH, AJ, AL, AN, BB, BD, BF, BH, BJ, BL, BN, CB, CD, CF, CH, CJ, CL, CN, DB, DD, DF, DH, DJ, DL, DN, EB, ED, EF, EH, EJ, EL, EN, FB, FD, FF, FH, FJ, FL, FN, GB, GD, GF, GH, GJ, GL, GN, HB, HD, HF, HH, HJ, HL, HN, IB, ID, IF, IH, IJ, IL, IN, JB, JD, JF, JH, JJ, JL, JN, KB, KD, KF, KH, KJ, KL, KN, LB, LD, LF, LH, LJ, LL, LN, MB, MD, MF, MH, MJ, ML, MN, NB, ND, NF, NH, NJ, NL, NN, OB, OD, OF, OH, OJ, OL, ON, PB, PD, PF, PH, PJ, PL, PN, QB, QD, QF, QH, QJ, QL, QN, RB, RD, RF, RH, RJ, RL, RN, SB, SD, SF, SH, SJ, SL, SN, TB, TD, TF, TH, TJ, TL, TN, UB, UD, UF, UH, UJ, UL, UN, VB, VD, VF, VH, VJ, VL, VN, WB, WD, WF, WH, WJ, WL, WN
Length: 443 amino acids
Theoretical weight: 49.62 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: P41352 (Residues: 1-443; Coverage: 100%)
Gene names: BTU1, BTU2
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 161 x GTP
Ligand MG 161 x MG
Ligand GDP 161 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-40436

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