› 8sfo

Electron Microscopy
3.3Å resolution

WT CRISPR-Cas12a with a 20bp R-loop and nontarget strand in the RuvC active site.

Released:
DOI: 10.2210/pdb8sfo/pdb
PDB entry 8sfo coloured by chain, front view.
PDB entry 8sfo coloured by chain, side view.
PDB entry 8sfo coloured by chain, top view.
Source organisms:
Entry authors: Strohkendl I, Taylor DW
Related structures: EMD-40446

Function and Biology Details

Reactions catalysed: 
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
RNA = a 5'-hydroxy-ribonucleotide + n nucleoside-2',3'-cyclophosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270725 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas12a Chain: A
Molecule details ›
Chain: A
Length: 1311 amino acids
Theoretical weight: 151.71 KDa
Source organism: Acidaminococcus sp. BV3L6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: U2UMQ6 (Residues: 1-1307; Coverage: 100%)
Gene names: HMPREF1246_0236, cas12a, cpf1
Sequence domains:
DNA (40-MER) Chain: C
Molecule details ›
Chain: C
Length: 56 nucleotides
Theoretical weight: 17.21 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (35-MER) Chain: D
Molecule details ›
Chain: D
Length: 56 nucleotides
Theoretical weight: 17.3 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (39-MER) Chain: B
Molecule details ›
Chain: B
Length: 48 nucleotides
Theoretical weight: 15.35 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-40446
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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